Antibacterial susceptibility assay agar well diffusion assay perez et al. In vitro antibacterial activity of selected tanzania. Plants have an almost limitless ability to synthesize aromatic substances, most of which are phenols or their oxygensubstituted derivatives. Methanol, ethylacetate extracts of plant species heracleum crenatifolium, h. Antibacterial screening suitable incubation, growth will occur in those tubes the antibacterial assay was performed by disc diffusion technique and minimum inhibitory concentration. In the present investigation, the active leaf extract of six medicinally important plants obtained by. Antibacterial activity of essential oils and plant extracts of artemisia artemisia annua l. Antibacterial, antifungal and antioxidant activities of some. Antibacterial activity, urinary tract infection, antibiotics, punica granatum.
This plant has been reported to have insecticidal, antifungal, antioxidant,14 molluscicidal,15 and antibacterial activity. Biochemistry graduates to have results from their final year. Pdf antibacterial activity of some plant extracts researchgate. Pdf antibacterial and antifungal activity of plant extracts. Minimum inhibitory concentrations of medicinal plants used. Antibacterial and antifungal activity of plant extracts article pdf available in rocznik ochrona srodowiska 171. Staphylococcus epidermis, which can cause wound and urinary tract infection, was inhibited by cassia alata.
The extract was kept in oven at 500c for 1hrs to evaporate excess ethanol and water. Antibacterial activity of medicinal plants used against. The antibacterial activity was tested against five bacterial strains by agar well diffusion method. Antibacterial screening of plant extract as shown by the inhibition zone diameter bacterial strain inhibition zone mm h. The present study was undertaken to investigate the antibacterial activity of the plant extract potentiating the same for some antibiotics in combination. The results showed that 16 extracts have antibacterial activity with more than 10 mm against a. Antibacterial effects of banana pulp extracts based on. Antibacterial and antifungal activities of thespesia populnea. Antibacterial activity of essential oils and plant extracts. A high activity of rockrose species against all bacterial and fungal test strains compared with reference agents gentamycin for bacteria and.
In general, the antibiotic controls produced greater zones of inhibition except for povidone iodine 10% which antibacterial activity was comparable to the 100% extract on s. Antibacterial activity of leaf extracts of biophytum. The extract was filtered using what man filter paper no. Introduction foodborne pathogenic are group of microorganisms that cause foodborne disease thus, the research for. Antibacterial activity was determined by the well diffusion method. Almost each and every part of this plant has been utilized for biological applications. Antibacterial and antifungal activities from leaf extracts. There is also evidence of antibacterial activity of musa sapientum leaves extract invitro5.
The highest yield of plant extract was obtained from punica granatum 4. The antibacterial plant extract use natural herb as raw materials. Antibacterial, muscle relaxant, and hypnotic effects of. Antibacterial and antifungal activities from leaf extracts of. In many cases, these substances serve as plant defense.
Antibacterial activity of five medicinal plant extracts. Results the ethanolic extracts of 51 species inhibited escherichia coli, and 114 ethanolic extracts inhibited staphylococcus aureus. Total extractives of four of the five plants examined showed activity against staphylococcus aureus, bacillus subtilis, escherichia coli and pseudomonas aeruginosa. Department of environment health, school of public health. The growth of bacillus subtilis was inhibited by 36% of the plant extracts, which include cassia alata, c. Hot hexane extract showed an inhibition zone of 12 mm against. Antibacterial and antifungal activities of crude extracts were determined by disc diffusion method. Harmine has previously been identified as possessing antibacterial activities against several species of bacteria. It is therefore recommended that the nature and the number of the active antibacterial principles involved in each plant extract be studied in detail. Antibacterial plant extract manufacturers and supplier. Extraction of the calyptranthes concinna essential oil was carried out using. Samples of plant extracts or pure compounds can be added to fungal spores collected from solid cultures, placed on glass slides, and incubated at an.
Antibacterial activity of the plant extract of symphytum officinale l. Nabi shariatifar1, ayub ebadi fathabad2, gholamreza jahed khaniki1 and hassan gandomi nasrabadi3 1. Evaluation of antibacterial activity of these plant extracts was recorded in table 2 and illustrated in fig. Screening of indian plant extracts for antibacterial activity. The decrease in cytopathic effect in the presence of the plant extract is then measured. The antibacterial analysis of crude methanolic extracts showed the significant activity against the microbes.
Assessment of antibacterial activity of plant extracts. Antimicrobial activity of crude extracts of three medicinal. The plant extracts showing antibacterial activity can be further subjected to isolation of therapeutic antimicrobials and can carry out further pharmacological evaluation. The antibacterial activity of various preparations of aerva lanata plant extract was. Antibacterial evaluation of cuprous oxide nanoparticles. Emblica officinalis amla leaf extract potentiates p. The concentration of the bacterial suspension was adjusted to 0. Rebeccas data presence of a halo shows that the oil inhibits the growth of the bacteria. Grapeseed sage lemon balm neem pine grapeseed grapeseed grapeseed tea tree emmas data aiyshas data. Pdf antibacterial activity of medicinal plant extracts researchgate. Antibacterial, antifungal and antioxidant activities of.
The airdried plant samples were into uniform powder using milling machine separately. Study on antibacterial and antioxidant activity of. Antibacterial and antifungal activities of plant part extracts against four pathogenic bacteria two grampositive and negative and three pathogenic fungi were investigated by the agar disk diffusion method. The antibacterial activity of selected plants towards. Antimicrobial screening of medicinal plants popularly used in mato.
Antibacterial and antifungal activities of thespesia. Department of microbiology, faculty of basic sciences, islamic azad university, lahijan, iran 2. Determination of total phenol content the total phenol content was determined by the procedure of skerget et al. For therapeutic effect evaluation, the logrank test as performed by prism5 for mac os x graphpad. Minimum inhibition concentration was observed with ethanol extract of s.
The agar were seeded with 24h culture of the microorganism which met the 0. Endemic plants can be a source of new active ingredients to fight antibiotic. Pdf the present study aimed at evaluating the in vitro antimicrobial activity of methanolic. Each microorganisms were grown overnight at 37c in muellerhinton broth. The crude extract showed a broad spectrum of antibacterial activity by inhibiting both the gram positive and gram negative groups.
Mean zones of inhibition of different concentrations of neem leaf extract against s. Evaluation of antibacterial potential of selected plant. Biochemistry graduates to have results from their final. The resulting extracts were subsequently weighted to produce 0. Medicinal plants, conyza bonariensis, antimicrobial, mato grosso, acute toxicity. The leaves of anthonotha macrophylla which are widely used in folkloric medicine in africa were evaluated for antibacterial activity and its toxicological profile. Antibacterial activity of plant extracts 247 brazilian journal of microbiology 2000 31. Antimicrobial activity of some plant extracts against bacterial strains.
The residues both ethanolic and aqueous extract were. Antibacterial characheristics of plants from heracleum genus have relatively slightly been researched. The antimicrobial activity of ethanolic and water extracts of 141 plant species was determined using a deepwell broth microdilution method on commercially available bacterial strains. Antibiotic resistance is a serious problem worldwide. Evaluation of the antibacterial activity of essential oil. Assessment of antibacterial activity of neem plant. This study was based on screening antibacterial activity of the ethanol extract of baeckea frutescens l. Most are secondary metabolites, of which at least 12,000 have been isolated, a number estimated to be less than 10% of the total. In vitro antibacterial activity and phytochemical studies of. Antibacterial and cytotoxic activity of brazilian plant.
Extracts were first sterilized by sterile membrane syringe filter pore size 0. Concentrations of neem leaf extract against mssa figure 2. In addition, the possible synergistic effects when associated with antibiotics were studied. Medicinal plants have been used for centuries as remedies for. From the results of the disc diffusion screening, b. Preliminary studies have been conducted into the antibacterial activity of extracts from some local medicinal plants from the family connaraceae. The patterns of inhibition varied with the plant extract and the organisms tested.
Aqueous extract of neem leaf extract has a good therapeutic potential as anti hyperglycemic agent in iddm and niddm sonia bajaj, and srinivasan b. The antimicrobial activity of plant extracts and phytochemicals was evaluated with antibiotic susceptible and resistant microorganisms. It was found that three plant extracts from the 9 plants studied had antibacterial activity. Antimicrobial activity of five plant extracts were investigated against bacillus cereus. In vitro alireza massiha,1 mohammadreza majid khoshkholghpahlaviani, 2 khosro issazadeh, 1 sirus bidarigh, 3 saeed zarrabi4 1. Five plant species were investigated to evaluate their antibacterial activity against food poisoning bacteria including two strains of gram positive bacteria b.
Petri dishes containing mueller hinton agar are swabbed with a suspension about 108 cfuml on a mac farland scale. Plant extracts are a potentially useful source of antimicrobial compounds. The antibacterial potency of each plant extract was evaluated using five bacterial strains causing food poisoning diseases. Antibacterial activity of hypericum perforatum was determined by agar well diffusion method 19. The findings presented as minimum inhibition concentrations mic indicated that plant extracts possess varying antibacterial potencies as summarized in tables 1 and 2. Evaluation of the antibacterial activity of essential oil and aqueous and ethanolic extracts of quercus infectoria leaves on foodborne pathogenic bacteria. Ethanol extract six grams of the powdered plant material was extracted in a soxhlet extractor using 25ml of 40% ethanol.
The results from these investigations encourage that the plant extracts may be used as antiinfective agents. In vitro antibacterial activity and phytochemical studies. The results obtained from the present study provide evidence that ethanolic extracts of s. During this work, we used various hot and cold extraction techniques using distilled. This study fortifies that methanol and chloroform extracts found to be better antibacterial activity against all the test organisms than petroleum ether extract. Antibacterial activity of medicinal plant extracts article pdf available in brazilian journal of microbiology 384. It has antiinflammatory, antitumor, antispasmodic properties, and acts as an antioxidant. Antibacterial activity of essential oils and plant. Since many of the essential oils inhibited the growth of e. In addition to these assays, antifungal phytochemicals can be analyzed by a spore germination assay. Antibacterial activity of leaf extracts of baeckea frutescens. Plant material collection of calyptranthes concinna leaves was carried out.
Antibacterial activity of leaf extracts of baeckea. Antibacterial activity of methanolic extracts of terminalia. The antibacterial activity of aqueous and methanolic extract of amla emblica officinalis was determined against li and b. Another study on the extracts of pulp of musa sapientum var. Medicinal plants, plant extracts, antimicrobial activity, phytochemicals. The acetone extracts showed remarkable antibacterial activity. Pdf antibacterial activity of the plant extract of. Antibacterial activity of medicinal plants used against uti. The highest antibacterial activities at the lowest concentration were observed from organic extracts of s. The methanolic extract of caryophyllus aromaticus presented the highest antis.
Antibacterial, antifungal and antioxidant activities of some medicinal plants 2146 pak. In vitro antibacterial activity and in vivo therapeutic effect. Study on antibacterial and antioxidant activity of berberis. Plant extracts were prepared using distilled water and 50 % ethanol. Antibacterial activities of the leaves against clinical strains of staphylococcus aureus, escherichia coli, salmonella typhi, klebsiella pneumonia, streptococcus pyogenes and bacillus subtilis were compared. Antibacterial and antifungal activities of ethanol.
The present study aimed at evaluating the in vitro antimicrobial activity of methanolic extracts of some medicinal plants against escherichia coli, salmonella typhimurium, staphylococcus aureus and enterococcus sp. The plant extract concentrations prepared included 600 g ml and 900 g ml for antibacterial activity. Antibacterial and toxicological evaluation of the ethanol. Single component of antibacterial plant extract has high purity and obvious effect to be widely used in medcine and cleansers. Antimicrobial activity of some plant extracts against.
Material and methods plants material the leaves of pergularia daemia and. Phenolic content and antibacterial activity of extracts of. The antibacterial activity of the eight extracts were assayed in vitro by the agar microdilution method against three resistant bacteria. Antibacterial and antifungal activities of ethanol extracts. For the preparation of plant extracts, samples of a. In the study maximum inhibition was observed with ethanol extract of dicum against s. The results showed that extracts of mint, lemon balm and coriander among others inhibited the growth of e. Antibacterial and antifungal activity of flowers of. Plant extracts have shown inhibitory effect on the growth of the bacteria studied, although of distinct forms.
The highest antibacterial activity was exhibited by methanol cold extract against staphylococcus epidermidis 15 mm and bacillus cereus 14 mm respectively. Antibacterial activities of some borneo plant extracts. Cymbopogon citratus leaves and psidium guajava leaves were collected in may 2006 from an experimental field of the school of agronomical sciences. Aqueous extract, berberis vulgaris, antioxidant activity, anti bacterial activity. All plant extracts showed no antibacterial activity with their high mic values against esblproducing bacteria and cre. The extract of 50 g of dried plant materials with ethanol yielded plant extract residues ranged from 1.
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